4/2/2023 0 Comments X files home torrent![]() ![]() The lots provided are managed separately and qualified by additional functional validation. ![]() 72 Mb of data was generated, with an average genome coverage of 15.5 X, from approximately 0.5 Million 200 bp reads. coli (K12 MG1655 strain) genomic DNA using NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent. Representative Ion Torrent Run ReportĪ typical Ion Torrent run report for libraries made from E. Representative Results from Libraries Constructed Using NEBNext for Ion TorrentĠ.5 µg of DNA from 3 different genomes with varying GC content were used to construct 200 bp and 400 bp libraries using the NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent, analyzed by the Agilent® Bioanalyzer®. ![]() hands-on time.ĭNA Library Preparation Workflow for Ion Torrent The components and protocol for this kit have been optimized for the volumes and concentrations of DNA sheared by mechanical methods, and the kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. The NEBNext Fast DNA Library Prep Set for Ion Torrent is suitable when mechanical methods, such as Covaris ® acoustic shearing, are preferred for fragmentation of input DNA. ![]()
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